RRID:SCR_012429
The Illumina NextSeq550 is used for the generation of high-throughput sequencing data.
Applications include:
Image credit: Illumina.com
"The Fragment Analyzer systems utilize automated parallel capillary electrophoresis to provide reliable quality control (QC) for nucleic acids. With its unique design and intuitive features, common QC bottlenecks are resolved by the automation of key steps such as gel loading and sample injection increasing lab efficiency. A broad range of kits are available allowing easy qualification and quantification of DNA and RNA samples."
Text Credit: https://www.agilent.com/en/product/automated-electrophoresis/fragment-analyzer-systems
Arizona Genetics Core offers ABI7900 Cycler for Quantitative or Real-Time PCR. This instrument is configured to run 384-well plates only.
After a brief training, local users can schedule time on the instrument and run their own samples.
Arizona Genetics Core offers ABI7300 Cycler for Quantitative or Real-Time PCR. This instrument is configured to run 96-well plates only. After a brief training, local users can schedule time on the instrument and run their own samples. Alternatively, AZGC can provide qPCR and Real-Time PCR services for your lab.
Arizona Genetics Core offers high quality and fast turnaround fully automated DNA sequencing and Fragment Analysis services on multiple Applied Biosystems 3730 DNA Analyzers.
Arizona Genetics Core offers SNP genotyping on the Agena Bioscience MassARRAY® MALDI-TOF instrument. This instrument can run 96-well and 384-well chips. Using this technology we can multiplex SNPs for up to 36 loci. Contact Arizona Genetics Core to discuss SNPs of interest and multiplex design.
The Agilent Bioanalyzer 2100 offers quick and convenient sizing and quantification analysis for DNA or RNA.
The BioTek Synergy HT instrument empowers 96-well plate DNA and RNA fluorometric quantification. This service is available as a standalone QC or as part of a sample processing workflow.
AZGC operates the Kingfisher Flex Automated Extraction Platform. This instrument uses magnetic bead chemistry to isolate and purify DNA or RNA from a variety of input materials. Typical extractions are performed in a 96-well plate for maximum processing throughput. Select sample types including whole blood may benefit from a 24-well plate isolation, which can accommodate a higher input sample volume. Contact AZGC today to discuss your sample isolation requirements.
Downstream QC recommendations include a fluorometric sample quantification as well as a quality analysis.
Isolated DNA or RNA can be returned to researchers in plates or tubes or retained at AZGC for subsequent testing protocols.
Samples and materials are only stored at AZGC for 3 months following the completion of service. Materials not requested for return will be disposed of in keeping with our SOPs 90 days after service is complete.
Arizona Genetics Core offers 3 Cyclers for Quantitative or Real Time PCR. After a brief training, local users can schedule time on the instrument and run their own samples. Real Time PCR is also utilized as one of the platforms in our genotyping services.
Users provide their own consumables when using the instruments.
Real Time PCR provides a quantitative assessment of your sample for gene expression, gene transcription studies or targeted nucleic acids.
For a database of optimized Real-time PCR primers please click on the following link: http://www.realtimeprimers.org
Local users may browse our instruments available to schedule. To schedule an instrument, please use our iLab resource scheduler
Schedule time on an instrument-
Academic (UA): $27.00 | Academic (non-UA): $32.40| Industry: $40.50 Unit: Per Hour- Unassisted use
SNP Genotyping- Service-
Academic (UA): $5.00 | Academic (non-UA): $6.00 | Industry: $7.50 Unit: Per reaction*
Gene Expression- Service-
Academic (UA): $5.00 | Academic (non-UA): $6.00 | Industry: $7.50 Unit: Per reaction*
Varies by project
DNA Extraction
RNA Extraction
Taqman SNP Genotyping
Users will assume the cost (in-whole) of assay-specific consumables. Arizona Genetics Core is happy to order the materials from our preferred manufacturer at the most appropriate scale for your project.