"Covaris Adaptive Focused Acoustics (AFA) technology is firmly established as the fragmentation method of choice for NGS, and Covaris continues to innovate the tools required to shear DNA and RNA without GC bias or thermal damage.

The Covaris AFA process is conducted under isothermal conditions, ensuring the integrity of the nucleic acid sample is maintained and providing high recovery of double-stranded DNA during the shearing process.

Combined with the specifically engineered AFA Tubes it is possible to precisely and accurately fragment DNA and RNA to the 100 – 1500bp range (microTUBE), or 2 – 5kb range (miniTUBE)."

Text Credit:https://www.covaris.com/dna-rna-shearing-for-ngs/

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AZGC uses Covaris Ultrasonicator to shear genomic DNA prior to DNA Library Prep Protocols.

The Illumina NextSeq550 is used for the generation of high-throughput sequencing data.

Applications include:

• Whole Genome Sequencing (Shotgun/de novo)
• Metagenome Analysis
• CHiPseq
• miRNA profiling
• Exome Sequencing
• RNASeq (transcriptome sequencing)
• Single Cell Sequencing
• Other supported and custom protocols (please inquire)

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Image credit: Illumina.com

The Arizona Genetics Core offers Illumina MiSeq supported applications including DNASeq, RNAseq, targeted gene sequencing, Metagenomics (16S/custom), small genome sequencing, amplicon sequencing and other NGS pipelines.

DNA or RNA from a variety of sample types can be run on Illumina MiSeq including cells, tissue, and blood. We also offer DNA or RNA extraction for library prep.

Applications include:

• Small Genome Sequencing (Shotgun/de novo)
• Metagenome Analysis (16S/custom)
• CHiPseq
• miRNA profiling
• Cancer Genomics (RUO)
• RNASeq (transcriptome sequencing/expression analysis)
• Library QC
• Single Cell sequencing
• Other supported and custom protocols (please inquire)

"The Fragment Analyzer systems utilize automated parallel capillary electrophoresis to provide reliable quality control (QC) for nucleic acids. With its unique design and intuitive features, common QC bottlenecks are resolved by the automation of key steps such as gel loading and sample injection increasing lab efficiency. A broad range of kits are available allowing easy qualification and quantification of DNA and RNA samples."

Text Credit: https://www.agilent.com/en/product/automated-electrophoresis/fragment-analyzer-systems

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Arizona Genetics Core offers ABI7900 Cycler for Quantitative or Real-Time PCR. This instrument is configured to run 384-well plates only.

After a brief training, local users can schedule time on the instrument and run their own samples.

Arizona Genetics Core offers ABI7300 Cycler for Quantitative or Real-Time PCR. This instrument is configured to run 96-well plates only. After a brief training, local users can schedule time on the instrument and run their own samples. Alternatively, AZGC can provide qPCR and Real-Time PCR services for your lab.

Arizona Genetics Core offers high quality and fast turnaround fully automated DNA sequencing and Fragment Analysis services on multiple Applied Biosystems 3730 DNA Analyzers.

Arizona Genetics Core offers SNP genotyping on the Agena Bioscience MassARRAY® MALDI-TOF instrument. This instrument can run 96-well and 384-well chips. Using this technology we can multiplex SNPs for up to 36 loci. Contact Arizona Genetics Core to discuss SNPs of interest and multiplex design.

The Agilent Bioanalyzer 2100 offers quick and convenient sizing and quantification analysis for DNA or RNA.