AZGC will share project data with users via iLab Operations upon service completion. In the case of Next-Gen Sequencing data and Single Cell data, AZGC will transfer ownership to researchers using Cyverse, Globus or provide a physical hard drive containing your data. Once Next-Gen data ownership is accepted electronically or by accepting a physical data drive, AZGC will no longer possess a copy of this data.

Researchers are responsible for downloading and backing up data generated by our core. AZGC is not responsible for the retention of data beyond three months from the date of data generation.

All data generated using AZGC services will be shared with only the requesting researcher and their PI.  The data will not be presented without permission from the researchers.

The Arizona Genetics Core will keep project materials on-hand for 3 months after data is returned / project is completed. After 3 months, all samples and materials will be disposed of in keeping with our SOPs. If you would like to receive your materials back from us please arrange with Arizona Genetics Core during the project planning stages. If you have questions about this policy please contact our facility.

All samples provided to the Arizona Genetics Core (the facility) must be collected and transmitted in compliance with all applicable State, Federal, and Tribal laws, statutes, regulations, and rules. It is the responsibility of the submitting party to ensure all samples satisfy this requirement and by submitting work requests and related samples to the facility the submitter warrants that the collection and transmittal of all samples are in compliance with any applicable laws, statutes, regulations, and rules. If at any time, the facility learns that any sample is not in compliance with the above it reserves the right to discontinue any further work on said sample.

Listed Below are the primers that AZGC will supply free of additional charge. Please check the sequence against that of your vector to verify the sequence.

NOTE: Please type these primer names in uppercase when submitting your samples online for sequencing

• KS: CCT-CgA-ggT-CgA-Cgg-TAT-Cg
• M13F: TgT-AAA-ACg-ACG-gCC-AgT
• M13R: Agg-AAA-CAg-CTA-TgA-CCA-Tg
• PCM13F*: CgC-CAg-ggT-TTT-CCC-AgT-CAC-gAC
• PCM13R*: TCA-CAC-Agg-AAA-CAg-CTA-TgA-C
• SK: CCg-CTC-TAg-AAC-TAg-Tgg-ATC
• SP6: TTT-Agg-TgA-CAC-TAT-AgA-ATA
• T3: AAT-TAA-CCC-TCA-CTA-AAg-gg
• T7: TAA-TAC-gAC-TCA-CTA-TAg-gg

*PCM13F and PCM13R are the same as pUCM13F and pUCM13R

ORP Core Facilities
University of Arizona
PO BOX 210240
Tucson, AZ 85721

Email: CoreBusiness@email.arizona.edu

Taqman SNP Genotyping is a robust and economical method to type a small number of Single Nucleotide Polymorphisms, SNPs across a study population. This is appropriate for a few samples or several thousand.

General Information

Sample Input

Genomic DNA - Normalized to 5ng/µL

Users must provide their own SNP assays or we can order the assay and bill assay supplies to your project. AZGC does not stock Taqman Assays.

Expected Results

Results table listing the sample genotype detected at each polymorphic site.

Details

Sample Preparation Details

Sample input is gDNA normalized to 5ng/µL. We can receive your samples in 1.5-2.0mL tubes but we prefer 96-well plates. Samples which do not meet our input requirements will be subject to additional handling fees. Please contact our lab if you have questions about sample preparation.

Price

Academic (UA) $5.00 | Academic (non-UA): $6.00 | Industry: $7.50
Unit: Sample well

Contact AZGC to discuss your specific assay reagents.

Turnaround Time

Varies per project

Related Services

DNA Extraction, Quantification, Normalization

AZGC offers a 'Quick and Easy' DNA extraction as a low-cost option for certain sample types.

General Information

Sample Input

We provide you bar-coded tubes with buffer, add your sample and return to AZGC

Expected Results

Genomic DNA suitable for downstream testing in transgenic mouse assays or other robust screening assays.

Details

Sample Preparation Details

For mouse genotyping add 2-4mm of tail tip tissue directly to the buffer in the provided tubes. Seal tightly and return to AZGC.

Workflow

How to get started with your sample submission:

Log into iLab Operations Software and from AZGC's core page click Request Services

Video tutorial for iLab submission

*We will review your project request and select an extraction method prior to sample processing

Samples are taken directly from cell lysis to PCR saving time and resources.

Price

View AZGC Current Pricing

Academic (UA) $2.25 | Academic (non-UA): $2.70 | Industry: $3.38| Unit: Sample

Turnaround Time

2 business days- does not include downstream genotyping

Related Services

Transgenic Mouse Genotyping

Additional Information

This method forgoes certain quantification and purification steps when unnecessary for downstream processing.