Arizona Genetics Core

RRID:SCR_012429

Clean Up - PCR Products

AZGC offers purification of 96-well PCR products using EdgeBioSystems ExcelaPure PCR purification blocks. ExcelaPure 96-well UFC plates contain an optimized ultrafiltration membrane for high recovery of your PCR products.

General Information


Sample Input

To use this service, submit your templates in a 96-well reaction plate.

Expected Results

PCR product free from excess primers, dimers, DNTPs and salts which can interfere with DNA Sequencing.

Details


Sample Preparation Details

To use this service, submit your templates in a 96-well reaction plate.

The samples will need to be brought up to 100µL with molecular grade water.

To use this service as part of your DNA Sequencing workflow, simply check the radio button for "clean up" in the submission form when you submit your plate for High Volume Sequencing in iLab Operations Software.

Workflow

  • Log in to iLab Operations Software and click Request Services.
  • Record and attach the numbers at the end of your iLab Service ID to your samples and send or deliver to AZGC.
  • Upload your sample information

Excess primers, primer dimers, dNTPs and salts are filtered out under vacuum pressure, while your purified PCR product is retained on the ultrafiltration membrane.

Price

See AZGC current pricing

Academic (UA): $80.00 | Academic (non-UA): $96.00 | Industry: $120.00 Unit: 96-well Plate

Turnaround Time

3 days

Related Services

DNA Sequencing Quantification and Normalization PCR Products

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