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Flow Cytometry Immune Monitoring Shared Resource

RRID:SCR_023432

BD FACSCanto II - flow cytometer

The FACSCanto II is equipped with 3 lasers (405nm, 488nm, 633nm), 8 fluorescence channels and 2 channels for Forward Scatter and Side Scatter. This instrument is an analysis instrument only. The FACSCanto II is best for DNA quantification in mammalian, plant or yeast cells.

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FLOW FACScanto
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BD FACSAria III - cell sorter and flow cytometer

The FACSAria III is equipped with 5 lasers (355nm, 405nm, 488nm, 561nm, 633nm), 17 fluorescence channels and 2 channels for Forward Scatter and Side Scatter. It is a state of the art cell sorter capable of physically separating 4 populations simultaneously. Sorted cells can be collected into tubes, plates, or onto slides. Typically staff will perform startup, QC, and sorting, but highly experienced LSR users may qualify to receive training in how to operate the FACSAria III unassisted.

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FLOW FACSaria
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BD LSR II - flow cytometer

The LSR II is equipped with 5 air-cooled lasers (355nm, 405nm, 488nm, 532nm, 640nm), 16 fluorescent channels and 2 channels for Forward Scatter and Side Scatter. It is a state of the art digital flow cytometer, containing octagon and trigon optical arrays, allowing increased signal sensitivity. This is a train-to-use instrument, contact the core staff for more information about training.

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FLOW FACSlsr
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