If your lab is in a time crunch, isn't ready to receive training, or you have a particularly challenging sample, our experienced staff are available to run your experiment for you.

The rate will be equal to instrument time (per hour) plus a staff labor charge (see iLab for "assisted use" rates, the rates vary depending on the instrument). There may be an additional set-up fee. We can provide a quote to estimate costs in advance of the service.

Choosing the right instrument for your experimental needs and the corresponding fluorescent labels that will work best with that instrument is best done in consultation with our staff. Please reach out to us and we are glad to help.

Each instrument in our core facility requires specific training by one of our staff before a user can be allowed to operate it by themselves. The exact length of training varies due to the complexity of the instrument and the previous experience of the new user. Training is charged at the the instrument's routine hourly rate plus a per hour staff labor fee (training rates for all of our instruments can be found in iLab). The staff member makes the determination as to when the user is ready to operate an instrument by themselves.

Training is often 1-2 sessions of 1.5 hours each. We limit the number of trainees to a maximum of two individuals from the same lab. We teach the trainee how to get the most of the instrument and often make suggestions about sample prep, alternate techniques, controls, etc.

Please contact us if you have any questions.

This instrument is useful for quantifying and checking quality on DNA, RNA, and protein samples.

The Cytation 1 is a key component of the Seahorse XFe96 plate reader. This system is capable of fluorescence detection and high contrast brightfield imaging and includes Gen5 Image+ Software, 4x objective, DAPI filter cube and 365 nm LED light source for DAPI with the ability to provide phenotypic and quantitative data in a single experiment

Applications include; cell migration and invasion assays, live cell imaging, label-free cell counting, multi-mode reader applications (including; immunofluorescence, phenotypic assays, ELISA plate reader)

Agilent Seahorse XFe96 Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate. Together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.

Standard assays include; real-time ATP rate, cell mito stress test, substrate oxidation stress test, glycolytic rate test, palmitate (fatty acid) oxidation stress test, mito fuel flex test, human T-cell activation, glycolytic stress test.

User training, staff-assisted use, or unassisted use are available for this instrument.