Flow Cytometry and Human Immune Monitoring Shared Resource FAQs

First, you need to know both the excitation and emission wavelengths of your chosen fluorophore. You can check both spectrums for many common fluorophores at the following spectrum viewers:
BD Spectrum Viewer
Life Technologies Spectrum Viewer
eBioscience Spectrum Viewer
BioLegend Spectrum Viewer

See the What lasers/detectors do you have? question below for more information on our instruments. If you have further questions, feel free to contact us at FlowCytometry@arizona.edu

We currently have several cytometers with a variety of lasers and detectors available for general use:

Individual consultation services are available to discuss applications and methodologies specific to your project. Contact the facility to set up an appointment (FlowCytometry@arizona.edu). There is also a small library of books and reference materials which are available to use.

Current rates can be found on our Rates page.

Flow cytometric analysis. Examples of assays include, but are not limited to: immunophenotyping, cell cycle analysis, DNA ploidy analysis, apoptosis/autophagy/necrosis, cell proliferation (BrdU incorporation), intracellular antigen/protein measurement and cytokine detection (bead assay), membrane potential measurement, intracellular pH/calcium (Ca++ flux) analysis, and oxidative stress detection.

High-speed aseptic cell sorting. Cell sorting based on cell surface marker immunostaining and/or side population staining. Our sorter is capable of sorting rare events or side-population cells. Example applications include isolation of tissue stem cells and cancer stem-like cells, isolation of fluorescently labeled cells in a mixed population, and isolation of cells within specific stages of the cell cycle.