The ImageStreamX Mk II is a flow cytometer which uses digital cameras to record fluorescence images of each cell from suspension, instead of recording only fluorescence intensity from each cell. Because these images record fluorescence location -- co-localization, translocation, and morphology can now be measured for large numbers of individual cells -- making even rare event analysis possible. Four lasers (405nm, 488nm, 561nm, 642nm) enable 10 fluorescence channels as well as classic brightfield channels. Sophisticated image analysis algorithms allow for discrimination of cellular processes with statistical significance. Can be used to determine colocalization, translocation, and morphology for large numbers of cells, with the possibility of rare event analysis.
Please acknowledge NIH award S10 OD028466 in publications which use data from this instrument, which was secured by the diligent work of Dr. Deepta Bhattacharya.
To use this instrument please contact the core via email to set an appointment.
