Flow Cytometry Immune Monitoring Shared Resource

RRID:SCR_023432

Complex immune assays

Our Shared Resource can provide the needed instrumentation, expertise and support for assays such as: T cell proliferation & activation, ELISA, functional phenotyping, genomic immune profiling and more. Ask for more details.

Service

Staff assistance - specific instruments

If your lab is in a time crunch, isn't ready to receive training, or you have a particularly challenging sample, our experienced staff are available to run your experiment for you.

The rate will be equal to instrument time (per hour) plus a staff labor charge (see iLab for "assisted use" rates, the rates vary depending on the instrument). There may be an additional set-up fee. We can provide a quote to estimate costs in advance of the service.

Service

Flow cytometry panel design

Choosing the right instrument for your experimental needs and the corresponding fluorescent labels that will work best with that instrument is best done in consultation with our staff. Please reach out to us and we are glad to help.

Service

Instrument training - specific to each instrument

Each instrument in our core facility requires specific training by one of our staff before a user can be allowed to operate it by themselves. The exact length of training varies due to the complexity of the instrument and the previous experience of the new user. Training is charged at the the instrument's routine hourly rate plus a per hour staff labor fee (training rates for all of our instruments can be found in iLab). The staff member makes the determination as to when the user is ready to operate an instrument by themselves.

Training is often 1-2 sessions of 1.5 hours each. We limit the number of trainees to a maximum of two individuals from the same lab. We teach the trainee how to get the most of the instrument and often make suggestions about sample prep, alternate techniques, controls, etc.

Please contact us if you have any questions.

Service
Contact

Nanodrop spectrophotometer

This instrument is useful for quantifying and checking quality on DNA, RNA, and protein samples.

Equipment

BioTek Cytation 1 plate reader

The Cytation 1 is a key component of the Seahorse XFe96 plate reader. This system is capable of fluorescence detection and high contrast brightfield imaging and includes Gen5 Image+ Software, 4x objective, DAPI filter cube and 365 nm LED light source for DAPI with the ability to provide phenotypic and quantitative data in a single experiment

Applications include; cell migration and invasion assays, live cell imaging, label-free cell counting, multi-mode reader applications (including; immunofluorescence, phenotypic assays, ELISA plate reader)

Image
cytation RT monitor
Equipment