Campus users of the Biacore T200 SPR system should consider using BLI as an alternative.
The Octet RED384 is a non-fluorescence-based, high-throughput, bench top, protein interaction detection system that uses Bio-Layer Interferometry (BLI) technology, and can analyze up to 16 samples in parallel for affinity, kinetics or active protein concentration determination from samples in 96- or 384-well microplates. It is a powerful detection platform for kinetic measurements & quantitation using the widest array of label-free binding assays available including ready made, custom coated, Biosensors:
Streptavidin
Protein A
Protein G
Protein L
Ni-NTA
Anti Penta-Histidine
Anti-GST
Anti-Human-Fab(CH1)
Amine Reactive (NHS-EDC)
Amino-Propyl-Silane
Anti-Mouse
Anti-Human
The closest technology to BioLayer Interferometry (BLI) is Surface Plasmon Resonance (SPR). While these technologies can produce similar data sets, BLI has advantages for certain aplications. The principal advantages of BLI are:
Its ability to analyze crude samples with a wider range of chemicals present (glycerol, DMSO, etc…)
The sample processing is fluidics free, enabling samples to be reused for multiple subsequent assays (it is a not destructive/consumptive process).
The Octet RED384 also has a greatly increased throughput over SPR instruments, which makes it ideal for high-throughput screening projects.
The Octet RED384 16-channel instrument provides analytical performance similar to the 8- channel Octet RED96 system, but with higher levels of throughput, speed, and flexibility. The high detection sensitivity and impressive throughput of Octet RED384 system has made it a favorite for small molecule library screening and analysis of large cross-competition binding matrices.
For best results on the Octet use Greiner 96 well plate 655209 or Greiner 384 well plate 781209 (plates are available for purchase in the core)
Facility Email: fgc@arizona.edu
