ICEL research, education and collaboration activities between July 1, 2021 and May 1, 2023.

Research

 The Imaging Cores - Electron is a unique full-service lab at the University of Arizona, providing access to advanced expertise in Biological Transmission Electron Microscopy (TEM) to support basic and translational research, from consultation to high-resolution imaging, data analysis and interpretation. The core facility house the FEI Tecnai G2 Spirit BT transmission electron microscope and several ultramicrotomes, enabling investigators the ultrastructural evaluation and analysis of biological and non-biological samples, serving the academy and industry.

Education

 The core facility provides access to hands-on TEM instrument and ultramicrotomy training. From a total of 86 users, 54.35 % training and assisted use were provided to graduate students, postdoctoral fellow, research staff and  faculty. 

Grants

 Two grants were supported.

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Publications

2023

 Barefield DY, Tonino P, Woulfe KC, Rahmanseresht S, O'Leary TS, Burnham HV, Wasserstrom JA, Kirk JA, Previs MJ, Granzier HL, McNally EM. Myosin-binding protein H-like regulates myosin-binding protein distribution and function in atrial cardiomyocytes. Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2314920120. doi: 10.1073/pnas.2314920120. Epub 2023 Dec 13. PMID: 38091294. pdf

Hurtado KA, Janda J, Schnellmann RG. Lasmiditan restores mitochondrial quality control mechanisms and accelerates renal recovery after ischemia-reperfusion injury. Biochem Pharmacol. 2023 Oct 20:115855. doi: 10.1016/j.bcp.2023.115855. pdf

Thota, L.N.R.; Rosales, J.L.; Placencia, I.; Zemskov, E.A.; Tonino, P.; Michael, A.N.; Black, S.M.; Chignalia, A.Z. The Pulmonary Endothelial Glycocalyx Modifications in Glypican 1 Knockout Mice Do Not Affect Lung Endothelial Function in Physiological Conditions. Int J Mol Sci. 2023, 24(19), 14568. pdf

Makam S, Sétamou M, Alabi OJ, Day W, Cromey D, Nwugo C. Mitigation of Huanglongbing: Implications of a biologically-enhanced nutritional program on yield, pathogen localization and host gene expression profiles. Plant Dis. 2023 Jul 6. doi: 10.1094/PDIS-10-22-2336-RE. pdf 

2022

Roxas JL, Ramamurthy S, Cocchi K, Rutins I, Harishankar A, Agellon A, Wilbur JS, Sylejmani G, Vedantam G, Viswanathan VK. Enteropathogenic Escherichia coli regulates host-cell mitochondrial morphology. Gut Microbes. 2022 Jan-Dec;14(1):2143224. doi: 10.1080/19490976.2022.2143224. pdf

Lindqvist J, Kolb J, de Winter J, Tonino P, Hourani Z, Labeit S, Ottenheijm C, Granzier H. Removal of MuRF1 Increases Muscle Mass in Nemaline Myopathy Models, but Does Not Provide Functional Benefits. Int J Mol Sci. 2022 Jul 23;23(15):8113. doi: 10.3390/ijms23158113. pdf

Sivinski J, Ngo D, Zerio CJ, Ambrose AJ, Watson ER, Kaneko LK, Kostelic MM, Stevens M, Ray AM, Park Y, Wu C, Marty MT, Hoang QQ, Zhang DD, Lander GC, Johnson SM, Chapman E. Allosteric differences dictate GroEL complementation of E. coli. FASEB J. 2022 Mar;36(3):e22198. doi: 10.1096/fj.202101708RR. PMID: 35199390; PMCID: PMC8887798. pdf

Jandova J, Park SL, Corenblum MJ, Madhavan L, Snell JA, Rounds L, Wondrak GT. Mefloquine induces ER stress and apoptosis in BRAFi-resistant A375-BRAF^V600E /NRAS^Q61K malignant melanoma cells targeting intracranial tumors in a bioluminescent murine model. Mol Carcinog. 2022 Jun;61(6):603-614. doi: 10.1002/mc.23407. Epub 2022 Apr 13. PMID: 35417045; PMCID: PMC9133119. pdf

2021

Biquand A, Spinozzi S, Tonino P, Cosette J, Strom J, Elbeck Z, Knöll R, Granzier H, Lostal W, Richard I. Titin M-line insertion sequence 7 is required for proper cardiac function in mice. J Cell Sci. 2021 Sep 15;134(18):jcs258684. doi: 10.1242/jcs.258684. pdf

A. Biquand, S. Spinozzi, P. Tonino, J. Strom, J. Cosette, Z. Elbeck, R. Knöll, H. Granzier, W. Lostal, I. Richard, OTHER NMDs: EP.360 Is7 domain of titin is essential for cardiac function in mice. Neuromuscular Disorders, Volume 31, Supplement 1, 2021, Page S159, ISSN 0960-8966, doi.org/10.1016/j.nmd.2021.07.385. pdf

Anandhan, A., Nguyen, N., Syal, A., Dreher, L. A., Dodson, M., Zhang, D. D., & Madhavan, L. (2021). Nrf2 loss accentuates parkinsonian pathology and behavioral dysfunction in human α-synuclein overexpressing mice. Aging and Disease, 12(4), 964–982. pdf

Ahmed, N. S., Harrell, L. M., Wieland, D. R., Lay, M. A., Thompson, V. F., & Schwartz, J. C. (2021). Fusion protein EWS-FLI1 is incorporated into a protein granule in cells. RNA, 27(8), 920–932. pdf

Qi, G., Mi, Y., Shi, X., Gu, H., Brinton, R. D., & Yin, F. (2021). ApoE4 impairs neuron-astrocyte coupling of fatty acid metabolism. Cell reports, 34(1), 108572. pdf