Transmission electron microscopy in situ hybridization (TEM-ISH) enables the localization of specific sequences of nucleic acids at high resolution.

Immuno-electron microscopy (IEM) is a technique that enable the localization of antigens at the ultrastructural level by labeling them with specific antibodies conjugated to (nano)gold-particles. The labeling is visualized as an electron-dense deposit in the site of the antigen-antibody reaction. IEM can be performed using the pre-embedding or post-embedding methods.

Thick resin sections of 0.5 - 1 µm suitable for light microscopy and ultrathin sections for TEM.

The workflow of samples processing for TEM involves several steps: primary fixation (performed in your lab), post-fixation, dehydration, infiltration and embedding in resin (performed in the core). Then ultramicrotomy provides thick sections (0.3 - 1 µm) stained with Toluidine blue suitable for light microscopy, and ultrathin (70 - 90 nm) sections stained with uranyl acetate or KMnO₄ and lead citrate, ready for TEM imaging in the FEI Tecnai G2 Spirit BT transmission electron microscope.

The Leica Ultracut UCT Ultramicrotome is used for cutting thick and ultrathin resin sections, on either glass or diamond knifes, for TEM or SEM. Ultramicrotomy training is provided to users interested in obtaining their own thick and ultrathin sections.

The Reichert-Jung Ultracut E ultramicrotome is used for cutting thick and ultrathin resin sections, on either glass or diamond knifes, for TEM or SEM. Ultramicrotomy training is provided to users interested in obtaining their own thick and ultrathin sections.

The FEI Tecnai G2 Spirit BT transmission electron microscope is a general-purpose, high resolution instrument that enables the ultrastructural evaluation of biological and non-biological samples with bright-field, dark- field and diffraction modes of operation.
The features include:
20-120kV.
LaB6 gun.
Single-tilt specimen holder.
Magnifications 22 - 300 kX.
Side-mounted AMTXR41 (4MP) and bottom AMTXR80 (8MP) digital CCD cameras.