Quantitative analyses are performed previous discussion of the parameters to be analyzed on your samples, using the AMT Image Capture Engine tools, or commonly used free open source image analysis software available, such as Fiji/ImageJ, Cell Profiler, Ilastick, Orbit, Icy and Cytomine.

Individual training is available to the users interested in operating the FEI Tecnai G2 Spirit BT transmission electron microscope to obtain their own images.  The Assisted use mode is available to the users interested in the staff imaging their samples upon request. 

Individual training is available to the users interested in sectioning their samples in the ultramicrotomes. In the Assisted use mode the staff perform the sectioning (thick and ultrathin sections) samples upon request. 

The EMS100X glow discharge unit produces a plasma current of 0 - 50 mA with positive and negative polarity with respect to carbon film surface, 60 mm diameter and aluminium electrode.

Transmission electron microscopy in situ hybridization (TEM-ISH) enables the localization of specific sequences of nucleic acids at high resolution.

Immuno-electron microscopy (IEM) is a technique that enable the localization of antigens at the ultrastructural level by labeling them with specific antibodies conjugated to (nano)gold-particles. The labeling is visualized as an electron-dense deposit in the site of the antigen-antibody reaction. IEM can be performed using the pre-embedding or post-embedding methods.

Thick resin sections of 0.5 - 1 µm suitable for light microscopy and ultrathin sections for TEM.

The workflow of samples processing for TEM involves several steps: primary fixation (performed in your lab), post-fixation, dehydration, infiltration and embedding in resin (performed in the core). Then ultramicrotomy provides thick sections (0.3 - 1 µm) stained with Toluidine blue suitable for light microscopy, and ultrathin (70 - 90 nm) sections stained with uranyl acetate or KMnO₄ and lead citrate, ready for TEM imaging in the FEI Tecnai G2 Spirit BT transmission electron microscope.